HomeOnline CataloguePCRPCR – DNA Polymerases"Hot Start" PCR - "proofreading"

"Hot Start" PCR - "proofreading"

For robust, difficult & long PCR reactions strictly requiring "HotStart" conditions (e.g. fingerprinting with fluorescent detection, multiplex PCR etc.). Increased sensitivity, specificity, yield and robustness as compared to "plain"" HotStart" Taq DNA polymerases.


All Products in this category

on PfuPlus! DNA Polymerase

"Hot Start" Pfu DNA polymerase blend, chemical inhibition.
  • Robust, long "HotStart" PCR.
  • High "proofreading" activity.
  • Separate enzyme and 10x buffers.
  • Both, non-colored and colored 10x PCR buffers for direct gel loading.

Polymerase X — ON Hybrid DNA Polymerase

"Hot Start" Hybrid DNA Polymerase variant, chemical inhibition.
  • Enhanced processivity.
  • High "proofreading" activity.
  • Separate enzyme and 10x buffers.
  • Non-colored and colored 10x PCR buffers for direct gel loading.

Perpetual Opti Taq DNA Polymerase

"Hot Start" OptiTaq DNA polymerase (Taq+Pfu), MAB inhibited.
  • Robust, long "HotStart" PCR.
  • Moderate "proofreading".
  • Separate enzyme and 10x buffers.
  • Both, non-colored and colored 10x PCR buffers for direct gel loading.

Color Perpetual Opti Taq DNA Polymerase

"Hot Start" OptiTaq DNA polymerase (Taq+Pfu), MAB inhibited.
  • Robust, long "HotStart" PCR.
  • Moderate "proofreading".
  • Separate enzyme and 10x buffers.
  • Polymerase supplemented with two tracer dyes for direct gel loading.

TI Amplus DNA Polymerase

TI-inhibited "HotStart" Th.sp.+Py.sp. "proofreading" blend.
  • Extremely long PCR (up to 40 kb).
  • Moderate "proofreading".
  • Separate enzyme and 10x buffers.
  • Non-colored and colored 10x PCR buffers for direct gel load

TI Opti Taq DNA Polymerase

TI-inhibited "HotStart" Taq + Pyrococcus sp. DNA Polymerase for high yield, robust PCR amplification.
  • Robust, difficult & long PCR.
  • Moderate "proofreading".
  • Separate enzyme and 10x buffers.
  • Contains both, non-colored and colo