Perpetual Opti Taq DNA Polymerase
"Hot Start" OptiTaq DNA polymerase (Taq+Pfu), MAB inhibited.
- Robust, long "HotStart" PCR.
- Moderate "proofreading".
- Separate enzyme and 10x buffers.
- Both, non-colored and colored 10x PCR buffers for direct gel loading.
Detailed Product Description
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Application Example
Figure: Robust and sensitve: Short, medium and long range HotStart PCR amplifications with Perpetual OptiTaq DNA Polymerase (Cat. No. E2720) in a size range between ~1 and 20 kb. Molecular weight marker M1 (Perfect™ 1kb DNA Ladder). Lanes 1.1 to 20 kb: PCR amplification reactions (each reaction 1.25 U EURx Perpetual OptiTaq DNA Polymerase, Pol Buffer B, 0.2 mM dNTPs; 50 µl reaction volume).
Perpetual OptiTaq DNA Polymerase - Package Contents
PCR Logsheet
Figure: Robust and sensitve: Short, medium and long range HotStart PCR amplifications with Perpetual OptiTaq DNA Polymerase (Cat. No. E2720) in a size range between ~1 and 20 kb. Molecular weight marker M1 (Perfect™ 1kb DNA Ladder). Lanes 1.1 to 20 kb: PCR amplification reactions (each reaction 1.25 U EURx Perpetual OptiTaq DNA Polymerase, Pol Buffer B, 0.2 mM dNTPs; 50 µl reaction volume).
Perpetual OptiTaq DNA Polymerase - Package Contents
- Perpetual OptiTaq DNA Polymerase
- 10x Buffer Pol B (Standard reaction buffer containing MgCl2)
- 10x Buffer Pol C (Purple coloured reaction buffer, for direct gel loading)
- 10x Buffer Pol A (Optimization reaction buffer without MgCl2)
- 25 mM MgCl2 solution
- Same as Perpetual OptiTaq DNA Polymerase, plus ultrapure dNTP solution
PCR Logsheet