HomeOnline CataloguePCRPCR – DNA Polymerases"Hot Start" PCR - "proofreading" Perpetual Opti Taq DNA Polymerase

Perpetual Opti Taq DNA Polymerase

"Hot Start" OptiTaq DNA polymerase (Taq+Pfu), MAB inhibited.

  • Robust, long "HotStart" PCR.
  • Moderate "proofreading".
  • Separate enzyme and 10x buffers.
  • Both, non-colored and colored 10x PCR buffers for direct gel loading.

Detailed Product Description
 English Version
 Deutsche Version

Quantity Package Cat-No. Price in €
200 uE2720-0187.00
500 uE2720-04197.00
1000 uE2720-02344.00
5000 uE2720-031637.00
200 u / Kit (+ dNTPs)EK2720-0195.00
500 u / Kit (+ dNTPs)EK2720-04216.00
1000 u / Kit (+ dNTPs)EK2720-02383.00

Application Example

Figure: Robust and sensitve: Short, medium and long range HotStart PCR amplifications with Perpetual OptiTaq DNA Polymerase (Cat. No. E2720) in a size range between ~1 and 20 kb. Molecular weight marker M1 (Perfect™ 1kb DNA Ladder). Lanes 1.1 to 20 kb: PCR amplification reactions (each reaction 1.25 U EURx Perpetual OptiTaq DNA Polymerase, Pol Buffer B, 0.2 mM dNTPs; 50 µl reaction volume).

Perpetual OptiTaq DNA Polymerase - Package Contents
  • Perpetual OptiTaq DNA Polymerase
  • 10x Buffer Pol B (Standard reaction buffer containing MgCl2)
  • 10x Buffer Pol C (Purple coloured reaction buffer, for direct gel loading)
  • 10x Buffer Pol A (Optimization reaction buffer without MgCl2)
  • 25 mM MgCl2 solution
Perpetual OptiTaq DNA Polymerase - Kit Package Contents
  • Same as Perpetual OptiTaq DNA Polymerase, plus ultrapure dNTP solution
Additional Resources

 PCR Logsheet