TI Amplus DNA Polymerase

TI-inhibited "HotStart" Th.sp.+Py.sp. "proofreading" blend.

• Extremely long PCR (up to 40 kb).
• Moderate "proofreading".
• Separate enzyme and 10x buffers.
• Non-colored and colored 10x PCR buffers for direct gel load

  Quantity	Package	Cat-No.	Price in €
  	100 u / Kit (+ dNTPs)	EK2930-01	29.00
  	500 u / Kit (+ dNTPs)	EK2930-02	132.00
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  Application Example
  
  

  

  
  
  Figure: Very long range PCR with Amplus DNA Polymerase: PCR amplification of human chromosomal DNA (18, 22 24 kb) and of episomal DNA (phage λ). Marker DNA: Phage λ [GenBank J02459] digested with (MW1) HindIII, (MW2) KpnI and (MW3) ApaI, respectively.
  
  Additional Resources
  
   PCR Logsheet
  
   How to trigger Reverse Transcriptase Activity of Taq DNA Polymerase (Contributed Protocol)