Nucleases
For application in selective nucleic acid digestion reactions.
Home → Online Catalogue → Enzymes → NucleasesNucleasesFor application in selective nucleic acid digestion reactions. All Products in this categoryDNase I, RNase freeNonspecific deoxyribonuclease / endonuclease acting on ssDNA and dsDNA. For DNA-free RNA preparation and for generation of random DNA libraries.Exonuclease IssDNA specific, does not digest dsDNA. For easy removal of oligonucleotides from PCR reactions prior to sequencing.Exonuclease III ( E.coli )dsDNA specific, digests dsDNA at nicks producing single-stranded gaps, for construction of nested unidirectional deletions.Exonuclease T5Catalyzes the removal of nucleotides from dsDNA and ssDNA in the 5' to 3' direction.Lambda ExonucleaseActs specific on 5'-phosphorylated dsDNA while leaving the unphosphorylated strand intact.Mung Bean NucleaseUsed for mapping of transcripts; digests hairpin structures during cDNA synthesis, does not cleave opposite a nick.OMNI NucleaseNonspecific nuclease that completely degrades all forms of DNA and RNA (single- and double-stranded).RNase I (Ribonuclease I)Only available completely nonspecific ribonuclease that hydrolyzes phosphodiester bond after all four bases.RNase A (Ribonuclease A)RNase, endoribonuclease attacking 3' phosphate of pyrimidine nucleotidesRNase H (Ribonuclease H), E.coliEndonuclease, digests selectively RNA from DNA/RNA hybrids.S1 NucleaseNon-specific endonuclease, active on ssDNA and RNA, nicks, mismatches and gaps; for S1 mapping of nucleic acids.T7 Endonuclease IRecognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA and more slowly, nicked ds-DNA.RNase III (Ribonuclease III)Cleaves long double-stranded RNA (dsRNA) into short dsRNAs (13-30 bases), which, upon transfection, can induce RNAi. |