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Nucleases

For application in selective nucleic acid digestion reactions.


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DNase I – RNase free

Nonspecific deoxyribonuclease / endonuclease acting on ssDNA and dsDNA. For DNA-free RNA preparation and for generation of random DNA libraries.

Exonuclease I

ssDNA specific, does not digest dsDNA. For easy removal of oligonucleotides from PCR reactions prior to sequencing.

Exonuclease III ( E.coli )

dsDNA specific, digests dsDNA at nicks producing single-stranded gaps, for construction of nested unidirectional deletions.

Exonuclease T5

Catalyzes the removal of nucleotides from dsDNA and ssDNA in the 5' to 3' direction.

Lambda Exonuclease

Acts specific on 5'-phosphorylated dsDNA while leaving the unphosphorylated strand intact.

Mung Bean Nuclease

Used for mapping of transcripts; digests hairpin structures during cDNA synthesis, does not cleave opposite a nick.

OMNI Nuclease

Nonspecific nuclease that completely degrades all forms of DNA and RNA (single- and double-stranded).

RNase I (Ribonuclease I)

Only available completely nonspecific ribonuclease that hydrolyzes phosphodiester bond after all four bases.

RNase III (Ribonuclease III)

Cleaves long double-stranded RNA (dsRNA) into short dsRNAs (13-30 bases), which, upon transfection, can induce RNAi.

RNase A (Ribonuclease A)

RNase, endoribonuclease attacking 3' phosphate of pyrimidine nucleotides

RNase H (Ribonuclease H), E.coli

Endonuclease, digests selectively RNA from DNA/RNA hybrids.

S1 Nuclease

Non-specific endonuclease, active on ssDNA and RNA, nicks, mismatches and gaps; for S1 mapping of nucleic acids.