OMNI Nuclease
Nonspecific nuclease that completely degrades all forms of DNA and RNA (single- and double-stranded).
Detailed Product Description
English Version
Deutsche Version
General Description
OMNI Nuclease is a non-specific nuclease, catalyzing removal of all forms of nucleic acids (DNA and RNA; linear, circular, double and single stranded). The nuclease completely degrades nucleic acids to 5'-monophosphorate terminated oligonucleotides with a length of 2 – 6 nt.
Applications
Application Example
Figure 1: OMNI Nuclease activity test. Incubation of DNA (lanes 1 to 4) and RNA (lanes 5 to 8) with increasing amounts of OMNI nuclease. Digestion was performed at 37°C for 30 min. Digestion efficiency was monitored by 1% [w/v] agarose gel electrophoresis.
Quality Control / Purity Check
Figure 2: Purity check of OMNI nuclease on a 12% PAGE protein gel.
OMNI Nuclease is a non-specific nuclease, catalyzing removal of all forms of nucleic acids (DNA and RNA; linear, circular, double and single stranded). The nuclease completely degrades nucleic acids to 5'-monophosphorate terminated oligonucleotides with a length of 2 – 6 nt.
Applications
- Sample preparation for protein 2D electrophoresis
- Removal of nucleic acids contaminants from protein preparations
- Viscosity reduction in bacterial, yeast and mammalian protein extracts
Application Example
Figure 1: OMNI Nuclease activity test. Incubation of DNA (lanes 1 to 4) and RNA (lanes 5 to 8) with increasing amounts of OMNI nuclease. Digestion was performed at 37°C for 30 min. Digestion efficiency was monitored by 1% [w/v] agarose gel electrophoresis.
- Lanes 1-4: 37 µg Herring sperm DNA, with increasing amounts of OMNI Nuclease units from lane 1 to 4.
- Lanes 5-8: Torula Yeast RNA type VI, with increasing amounts of OMNI Nuclease units from lane 5 to 8.
- K-: Control lanes without OMNI Nuclease addition.
- MW: Molecular weight marker.
Quality Control / Purity Check
Figure 2: Purity check of OMNI nuclease on a 12% PAGE protein gel.
- Left lane: Protein molecular weight marker (numbers indicate MW in kDa).
- Right lane: 5 µg OMNI nuclease.