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Restriction Endonucleases

Blunt- and sticky end cleaving restriction endonucleases acting on dsDNA.

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AccI

Reaction temperature: 37°C ; recognition sequence: GT | MKAC ; Prototype: AccI

AcvI

Reaction temperature: 37°C ; recognition sequence: CAC | GTG ; Prototype: PmaCI

AluI

Reaction temperature: 37°C ; recognition sequence: AG | CT ; Prototype: AluI

ApaI

Reaction temperature: 25°C ; recognition sequence: GGGCC | C ; Prototype: ApaI

AvaI

Reaction temperature: 37°C ; recognition sequence: C | YCGRG ; Prototype: AvaI

AvaII

Reaction temperature: 37°C ; recognition sequence: G | GWCC ; Prototype: AvaII

BalI

Reaction temperature: 37°C ; recognition sequence: TGG | CCA ; Prototype: BalI

BamHI

Reaction temperature: 37°C ; recognition sequence: G | GATCC ; Prototype: BamHI

BanII

Reaction temperature: 37°C ; recognition sequence: GRGCY | C ; Prototype: HgiJII

BglI

Reaction temperature: 37°C ; recognition sequence: GCCNNNN | NGGC ; Prototype: BglI

BglII

Reaction temperature: 37°C ; recognition sequence: A | GATCT ; Prototype: BglII

BsiHKCI

Reaction temperature: 65°C ; recognition sequence: C | YCGRG ; Prototype: AvaI

BssHII

Reaction temperature: 50°C ; recognition sequence: G | CGCGC ; Prototype: BsePI

BstXI

Reaction temperature: 50°C ; recognition sequence: CCANNNNN | NTGG ; Prototype: BstXI

ClaI

Reaction temperature: 37°C ; recognition sequence: AT | CGAT ; Prototype: ClaI

CviJI

Reaction temperature: 37°C ; recognition sequence: RG | CY ; Prototype: CviJI

CviJI*

Reaction temperature: 37°C ; recognition sequence: G | C except YG / CR ; Prototype: CviJI*

DdeI

Reaction temperature: 37°C ; recognition sequence: C | TNAG ; Prototype: DdeI

DpnI

Reaction temperature: 37°C ; recognition sequence: GA | TC ; Prototype: DpnI

DraI

Reaction temperature: 37°C ; recognition sequence: TTT | AAA ; Prototype: AhaI

EcoRI

Reaction temperature: 37°C ; recognition sequence: G | AATTC ; Prototype: EcoRI

EcoRV

Reaction temperature: 37°C ; recognition sequence: GAT | ATC ; Prototype: EcoRV

FokI

Reaction temperature: 37°C ; recognition sequence: GGATG (9/13) ; Prototype: FokI

HaeIII

Reaction temperature: 37°C ; recognition sequence: GG | CC ; Prototype: HaeIII

HhaI

Reaction temperature: 37°C ; recognition sequence: GCG | C ; Prototype: HhaI

HincII

Reaction temperature: 37°C ; recognition sequence: GTY | RAC ; Prototype: HindII

HindIII

Reaction temperature: 37°C ; recognition sequence: A | AGCTT ; Prototype: HindIII

HinfI

Reaction temperature: 37°C ; recognition sequence: G | ANTC ; Prototype: HinfI

HpaI

Reaction temperature: 37°C ; recognition sequence: GTT | AAC ; Prototype: HpaI

HpaII

Reaction temperature: 37°C ; recognition sequence: C | CGG ; Prototype: HpaII

KpnI

Reaction temperature: 37°C ; recognition sequence: GGTAC | C ; Prototype: KpnI

MboI

Reaction temperature: 37°C ; recognition sequence: | GATC ; Prototype: MboI

MboII

Reaction temperature: 37°C ; recognition sequence: GAAGA (8/7) ; Prototype: MboII

MluI

Reaction temperature: 37°C ; recognition sequence: A | CGCGT ; Prototype: MluI

MmeI

Discontinued. Reaction temperature: 37°C ; recognition sequence: TCCRAC (20/18) ; Prototype: MmeI

MnlI

Reaction temperature: 37°C ; recognition sequence: CCTC (7/6) ; Prototype: MnlI

MspI

Reaction temperature: 37°C ; recognition sequence: C | CGG ; Prototype: HpaII

NarI

Reaction temperature: 37°C ; recognition sequence: GG | CGCC ; Prototype: NarI

NcoI

Reaction temperature: 37°C ; recognition sequence: C | CATGG ; Prototype: NcoI

NdeI

Reaction temperature: 37°C ; recognition sequence: CA | TATG ; Prototype: NdeI

NheI

Reaction temperature: 37°C ; recognition sequence: G | CTAGC ; Prototype: NheI

NotI

Reaction temperature: 37°C ; recognition sequence: GC | GGCCGC ; Prototype: NotI

NotI - reduced star activity

Reaction temperature: 37°C ; recognition sequence: GC | GGCCGC ; Prototype: NotI; reduced star activity

NruI

Reaction temperature: 37°C ; recognition sequence: TCG | CGA ; Prototype: NruI

NsiI

Reaction temperature: 37°C ; recognition sequence: ATGCA | T ; Prototype: NsiI

PinAI

Reaction temperature: 37°C ; recognition sequence: A | CCGGT ; Prototype: AgeI

PstI

Reaction temperature: 37°C ; recognition sequence: CTGCA | G ; Prototype: PstI

PvuI

Reaction temperature: 37°C ; recognition sequence: CGAT | CG ; Prototype: PvuI

PvuII

Reaction temperature: 37°C ; recognition sequence: CAG | CTG ; Prototype: PvuII

PvuII - reduced star activity

Reaction temperature: 37°C ; recognition sequence: CAG | CTG ; Prototype: PvuII

RsaI

Reaction temperature: 37°C ; recognition sequence: GT | AC ; Prototype: RsaI

RsrII

Reaction temperature: 37°C ; recognition sequence: CG | GWCCG ; Prototype: RsrII

SacI

Reaction temperature: 37°C ; recognition sequence: GAGCT | C ; Prototype: SacI

ScaI - reduced star activity

Reaction temperature: 37°C ; recognition sequence: GAGCT | C ; Prototype: SacI; reduced star activity

SacII

Reaction temperature: 37°C ; recognition sequence: CCGC | GG ; Prototype: SacII

SalI

Reaction temperature: 37°C ; recognition sequence: G | TCGAC ; Prototype: SalI

SalI - reduced star activity

Reaction temperature: 37°C ; recognition sequence: G | TCGAC ; Prototype: SalI; reduced star activity

Sau3AI

Reaction temperature: 37°C ; recognition sequence: | GATC ; Prototype: MboI

ScaI

Reaction temperature: 37°C ; recognition sequence: AGT | ACT ; Prototype: ScaI

SfiI

Reaction temperature: 50°C ; recognition sequence: GGCCNNNN | NGGCC ; Prototype: SfiI

SmaI

Reaction temperature: 25°C ; recognition sequence: CCC | GGG ; Prototype: SmaI

SpeI

Reaction temperature: 37°C ; recognition sequence: A | CTAGT ; Prototype: SpeI

SphI

Reaction temperature: 37°C ; recognition sequence: GCATG | C ; Prototype: SphI

SspI

Reaction temperature: 37°C ; recognition sequence: AAT | ATT ; Prototype: SspI

StuI

Reaction temperature: 37°C ; recognition sequence: AGG | CCT ; Prototype: StuI

TaqI

Reaction temperature: 65°C ; recognition sequence: T | CGA ; Prototype: TaqI

TaqII

Reaction temperature: 65°C ; recognition sequence: GACCGA (11/9) ; Prototype: TaqII

TspDTI

Reaction temperature: 70°C ; recognition sequence: ATGAA (11/9) ; Prototype: TspDTI

TspGWI

Reaction temperature: 70°C ; recognition sequence: ACGGA (11/9) ; Prototype: TspGWI

Tth111I

Reaction temperature: 65°C ; recognition sequence: GACN | NNGTC ; Prototype: Tth111I

XbaI

Reaction temperature: 37°C ; recognition sequence: T | CTAGA ; Prototype: XbaI

XhoI

Reaction temperature: 37°C ; recognition sequence: C | TCGAG ; Prototype: XhoI