ON Taq PCR Master Mix HOT START
Chemically inhibited "Hot Start" Taq DNA polymerase.
- Chemical inhibition.
- Taq inhibitor denaturation is irreversible.
- Taq contains specific, genetic modifications.
- Extremely tight "Hot Start".
Detailed Product Description
English Version
Deutsche Version
ON Taq PCR Master Mix (2x) - Package Contents
Figure 1: Application example, PCR amplification using ON TaqPCR Master Mix
- ON Taq PCR Master Mix (2x)
- PCR Water (Nuclease Free)
- 10x Color Load (Optinal reaction component: Coloured reaction buffer, for direct gel loading)
- Optionally supplied, if needed:
- 25 mM MgCl2 solution. If required, please request this component along with your order.
Figure 1: Application example, PCR amplification using ON TaqPCR Master Mix
- A 2 kb amplicon of the human β-globin gene was amplified using onTaq PCR Master Mix (2x). Just for the purpose of illustrating the effect of automatized "Hot Start", all PCR reactions were incubated 30 min at 25°C before PCR. Under these unfavorable conditions, formation of non-specific primer dimers / multimers and non-specific primer binding were forced. Since non-specific priming does not occur as long as Taq DNA polymerase is inhibited during room temperature incubation (i.e. during PCR setup), tight "Hot Start" is demonstrated by presence of only one specific band.
- Lane M: Molecular weight marker MW (→ Perfect™ 1kb DNA Ladder)
- Lane 1: PCR amplification reaction using non - "Hot Start" Taq PCR Master Mix (2x).
- Lanes 2, 3: PCR amplification reactions using ON Taq PCR Master Mix (2x).