Detailed Reference Manual (English Version Only)

Figure: Purification of scarce RNA from different human blood samples (400 µl each) and subsequent one-step RT-PCR of a human ArgI gene fragment (1.1 kb).
28S / 18S ratio of purified RNA is approx. 2:1, indicating high quality of purified RNA.
Enzymes: dART Reverse Transcriptase , Taq DNA Polymerase .
Ladder: Perfect Plus 1 kb DNA Ladder .
PCR conditions: 50°C-30 min, 94°C-5 min, (94°C-30 sec, 54°C-30 sec, 72°C -1 min) x 30 cycles and 72°C - 3min.

Links to Related Articles

• Universal Blood RNA Kit for fresh, frozen and preserved blood samples of human and animal origin
• dART cDNA Synthesis Kit with all required and specifically adjusted components for Reverse Transcription
• Reverse Transcriptases
• Ribonuclease Inhibitor
• Ribonuclease H ( E.coli ) for selective RNA digestion from DNA/RNA hybrids only
• SG qPCR Master Mixes (2x) for qPCR / RealTime PCR

Additional Resources

 RT Logsheet

 qPCR Logsheet