Application Example

Figure: Robust short, medium and long range PCR amplifications with OptiTaq DNA Polymerase (Cat. No. E2600) in a size range between ~1 and 20 kb. Molecular weight markers MW2 (Lambda-DNA [Phage λ, GenBank J02459] / Hind III) and MW1 (Perfect™ 1kb DNA Ladder). Lanes 1.1 to 20 kb: PCR amplification reactions.

Notes:

Color OptiTaq DNA Polymerase is supplemented with two tracing dyes (yellow and red). These dyes allow direct loading of PCR products to agarose gels. Additionally, these dyes provide an optical control for a complete mixing during reaction setup by visualizing the complete suspension of polymerase in the reaction mix. This may prove useful for less experienced users such as teaching classes and student courses. In this Color edition, these dyes are mixed directly to the polymerase . As a consequence, the Color enzyme edition does not allow the end user to run PCR reactions without addition of dyes.

Note, that uncoloured standard OptiTaq DNA Polymerase contains both dyes in a separate coloured buffer, giving you a choice to run both colored and uncolored PCR reactions. Thus, when looking for a 10x buffer / optional gel loading dye combination, experienced researchers will want to use Standard OptiTaq DNA Polymerase . Among colourless buffers, the standard enzyme package contains a purple coloured buffer Pol C. Purple buffer Pol C allows to load aliquots of PCR reactions directly on gel without the need to add a separate gel loading buffer.

Click here for a direct link to the OptiTaq DNA Polymerase product page.

Additional Resources

 PCR Logsheet