Application Example

Figure: Robust short, medium and long range PCR amplifications with OptiTaq DNA Polymerase (Cat. No. E2600) in a size range between ˜1 and 20 kb. Molecular weight markers MW2 (Lambda-DNA [Phage λ, GenBank J02459] / Hind III) and MW1 (Perfect™ 1kb DNA Ladder). Lanes 1.1 to 20 kb: PCR amplification reactions.

OptiTaq DNA Polymerase - Package Contents

• OptiTaq DNA Polymerase
• 10x Buffer Pol B (Standard reaction buffer containing MgCl₂)
• 10x Buffer Pol C (Purple coloured reaction buffer, for direct gel loading)
• 10x Buffer Pol A (Optimization reaction buffer without MgCl₂)
• 25 mM MgCl₂ solution

OptiTaq DNA Polymerase - Kit Package Contents

• Same as OptiTaq DNA Polymerase, plus ultrapure dNTP solution

Additional Resources

•  PCR Logsheet
•  Direct PCR amplification from mouse tails using OptiTaq - a simple, fast and non-expensive approach towards high throughput mouse genotyping (Contributed Protocol)