TI Taq PCR Master Mix HOT START
Cost-effective "Hot Start" Taq DNA polymerase.
- Thermal dependent inhibitor (TI).
- Pol. inhibitor denaturation is reversible.
- Comparable performance to Perpetual Taq at reduced costs.
Detailed Product Description
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TI Taq PCR Master Mix (2x) - Package Contents
Figure 1: Application example, PCR amplification using TI TaqPCR Master Mix
- TI Taq PCR Master Mix (2x)
- PCR Water (Nuclease Free)
- 10x Color Load (Optinal reaction component: Coloured reaction buffer, for direct gel loading)
- Optionally supplied, if needed:
- 25 mM MgCl2 solution. If required, please request this component along with your order.
Figure 1: Application example, PCR amplification using TI TaqPCR Master Mix
- A 2 kb amplicon of the human β-globin gene was amplified using TI Taq PCR Master Mix (2x). Just for the purpose of illustrating the effect of automatized "Hot Start", all PCR reactions were incubated 30 min at 25°C before PCR. Under these unfavorable conditions, formation of non-specific primer dimers / multimers and non-specific primer binding were forced. Since non-specific priming does not occur as long as Taq DNA polymerase is inhibited during room temperature incubation (i.e. during PCR setup), tight "Hot Start" is demonstrated by presence of only one specific band.
- Lane M: Molecular weight marker MW (→ Perfect™ 1kb DNA Ladder)
- Lane 1: PCR amplification reaction using non - "Hot Start" Taq PCR Master Mix (2x).
- Lanes 2, 3: PCR amplification reactions using TI Taq PCR Master Mix (2x).