Amplus PCR Master Mix
Th.sp.+Py.sp. "proofreading" blend.
- Extremely long PCR.
- Up to 40 kb amplicon length.
- Moderate "proofreading".
- Contains separate Color Load solution for direct gel loading.
Application Example
Figure: Very long range PCR with Amplus DNA Polymerase: PCR amplification of human chromosomal DNA (18, 22 24 kb) and of episomal DNA (phage λ). Marker DNA: Phage λ [GenBank J02459] digested with (MW1) HindIII, (MW2) KpnI and (MW3) ApaI, respectively.
Additional Resources
PCR Logsheet
How to trigger Reverse Transcriptase Activity of Taq DNA Polymerase (Contributed Protocol)
Figure: Very long range PCR with Amplus DNA Polymerase: PCR amplification of human chromosomal DNA (18, 22 24 kb) and of episomal DNA (phage λ). Marker DNA: Phage λ [GenBank J02459] digested with (MW1) HindIII, (MW2) KpnI and (MW3) ApaI, respectively.
Additional Resources
PCR Logsheet How to trigger Reverse Transcriptase Activity of Taq DNA Polymerase (Contributed Protocol)