Figure 1: Color Taq DNA Polymerase - Package Contents

• Color Taq DNA Polymerase (contains purple coloured dye mix, for direct gel loading)
• 10x Buffer Pol A (Optimization reaction buffer without MgCl₂)
• 25 mM MgCl₂ solution

Color Taq DNA Polymerase - Kit Package Contents

• Same as Taq DNA Polymerase, plus ultrapure dNTP solution

Application Example

Figure: PCR amplification with Taq DNA Polymerase (Cat. No. E2500) in a size range between ~1 and 15 kb. Molecular weight markers MW1 (Perfect™ 1kb DNA Ladder), MW2 (Lambda-DNA [Phage λ, GenBank J02459] / Hind III). Lanes 1.1 to 15 kb: PCR amplification reactions (each reaction 1.25 U EURx Taq DNA Polymerase, Pol Buffer B, 0.2 mM dNTPs; 50 µl reaction volume).

Notes:

Color Taq DNA Polymerase is supplemented with two tracing dyes (yellow and red). These dyes allow direct loading of PCR products to agarose gels. Additionally, these dyes provide an optical control for a complete mixing during reaction setup by visualizing the complete suspension of polymerase in the reaction mix. This may prove useful for less experienced users such as teaching classes and student courses. In this Color edition, these dyes are mixed directly to the polymerase . As a consequence, the Color enzyme edition does not allow the end user to run PCR reactions without addition of dyes.

Note, that uncoloured standard Taq DNA Polymerase contains both dyes in a separate coloured buffer, giving you a choice to run both colored and uncolored PCR reactions. Thus, when looking for a 10x buffer / optional gel loading dye combination, experienced researchers will want to use Standard Taq DNA Polymerase . Among colourless buffers, the standard enzyme package contains a purple coloured buffer Pol C. Purple buffer Pol C allows to load aliquots of PCR reactions directly on gel without the need to add a separate gel loading buffer.

Click here for a direct link to the Taq DNA Polymerase product page.

Additional Resources

 PCR Logsheet

 How to trigger Reverse Transcriptase Activity of Taq DNA Polymerase (Contributed Protocol)