Fast Probe qPCR Master Mix
Sensitive, high precision, fast qPCR master mix for probe-based quantitative real-time PCR and two-step RT-qPCR. Plus separate UNG and PCR water. No ROX.
Detailed Product Description
English Version
Deutsche Version
Application Example
Figure 1: Performance comparison between EURx Probe qPCR Master Mix (2x) and Fast Probe qPCR Master Mix (2x). Fluorescent probe based real-time PCR was performed by using primers and a FAM-labeled probe specific for TUBB (beta-tubulin). Both, Fast Probe and “Standard” Probe qPCR reactions were performed by a 2-step cycling protocol. Extension time was 30 sec for Fast Probe, and 60 sec for “Standard” Probe qPCR reactions, resulting in a 40% time saving for Fast Probe vs. “Standard” Probe qPCR reactions.
Reactions were run in duplicate using human leukocyte cDNA (10 ng and 0.2 ng) as template, respectively. CT of reactions with equivalent cDNA template concentrations were very similar, respectively, and differed by max. 0.5 between Fast Probe qPCR and “Standard” Probe qPCR assays. For each corresponding reaction, duplicates showed near identical amplification plots. Thus, for each template concentration, amplification plots for one of both duplicates is displayed, respectively. A ~90% PCR efficiency was measured in all assays.
Probe qPCR Master Mix (2x) - Package Contents
qPCR Master Mix Logsheet
Figure 1: Performance comparison between EURx Probe qPCR Master Mix (2x) and Fast Probe qPCR Master Mix (2x). Fluorescent probe based real-time PCR was performed by using primers and a FAM-labeled probe specific for TUBB (beta-tubulin). Both, Fast Probe and “Standard” Probe qPCR reactions were performed by a 2-step cycling protocol. Extension time was 30 sec for Fast Probe, and 60 sec for “Standard” Probe qPCR reactions, resulting in a 40% time saving for Fast Probe vs. “Standard” Probe qPCR reactions.
Reactions were run in duplicate using human leukocyte cDNA (10 ng and 0.2 ng) as template, respectively. CT of reactions with equivalent cDNA template concentrations were very similar, respectively, and differed by max. 0.5 between Fast Probe qPCR and “Standard” Probe qPCR assays. For each corresponding reaction, duplicates showed near identical amplification plots. Thus, for each template concentration, amplification plots for one of both duplicates is displayed, respectively. A ~90% PCR efficiency was measured in all assays.
Probe qPCR Master Mix (2x) - Package Contents
- 2x Master Mix containing
- Perpetual Taq DNA Polymerase (Hot Start) ,
- probe optimized reaction buffer,
- dNTPs (dTTP is partially replaced with dUTP).
- Uracil-N-Glycosylase,
- H2O, PCR grade.
- 25 mM MgCl2 solution. If required, please request this component along with your order.
- SG qPCR Master Mix (2x) plus (10x) ROX Solution
- AMV Reverse Transcriptase Native
- Reverse Transcriptases
- Ribonuclease Inhibitor
- RNA Extraction & Purification Kits
qPCR Master Mix Logsheet