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Opti Taq Master Mix

Taq+Pfu "proofreading" blend.

  • Robust, difficult & long PCR.
  • Up to 20 kb amplicon length.
  • Moderate "proofreading"..
  • Contains separate Color Load solution for direct gel loading.

Detailed Product Description
 English Version
 Deutsche Version

Quantity Package Cat-No. Price in €
100 reactions
(100 x 50 μl)		E2910-0139.00
200 reactions
(200 x 50 μl)		E2910-0272.00
500 reactions
(500 x 50 μl)		E2910-03143.00



Application Example



Figure 1: PCR amplification using EURx OptiTaq Master Mix and EURx OptiTaq DNA Polymerase (separate components), respectively.

A 2.5 kb PCR amplicon was amplified with OptiTaq DNA Polymerase either in stand-alone or in master mix format, respectively.
  • Lane MW (1): molecular size marker- Perfect 1 kb DNA Ladder (Cat. No. E3130).
  • Lanes "Separate Components" (2,3): PCR amplification reactions using 1.25 U OptiTaq DNA Polymerase, Pol Buffer B and dNTPs.
  • Lanes "Master Mix" (4,5): PCR amplification reactions using OptiTaq PCR Master Mix (2x), after 25 freeze-thaw cycles.
  • Lanes "Master Mix + Dye" (6,7): PCR amplification reactions using OptiTaq PCR Master Mix (2x) and 10 x Color Load, after 25 freeze-thaw cycles.
Result: Following 25 freeze-thaw cycles, OptiTaq Master Mix still displayed similar performance to OptiTaq DNA Polymerase with separately stored components and not subjected to repeated freeze-thawing.



Figure 2: Robust short, medium and long range PCR amplifications with OptiTaq DNA Polymerase (Cat. No. E2600) in a size range between ~1 and 20 kb. Molecular weight markers MW2 (Lambda-DNA [Phage λ, GenBank J02459] / Hind III) and MW1 (Perfect™ 1kb DNA Ladder). Lanes 1.1 to 20 kb: PCR amplification reactions.

OptiTaq DNA Polymerase - Package Contents
  • OptiTaq Master Mix (2x)
  • PCR Water
  • 10x Color Load (optional buffer, for direct gel loading)
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