Probe qPCR Master Mix
Sensitive, high precision (2x) master mix for probe-based quantitative real-time PCR and two-step RT-qPCR. Plus separate UNG and PCR water. No ROX.
Detailed Product Description
English Version
Deutsche Version
Application Example
Figure 1: Probe-based real-time PCR plus UNG pretreatment with EURx Probe qPCR Master Mix (2x), GAPDH specific primers and a HEX-labeled probe binding within the amplicon. Reactions were performed in duplicates by using human leukocyte cDNA (10 ng and 0.2 ng) as template. Measured CT values as well as amplification plots were near identical for all corresponding duplicates, respectively. For each template DNA amount, amplification plots for one of both duplicates is displayed, respectively. Measured PCR efficiency was 98%.
Probe qPCR Master Mix (2x) - Package Contents
qPCR Master Mix Logsheet
Figure 1: Probe-based real-time PCR plus UNG pretreatment with EURx Probe qPCR Master Mix (2x), GAPDH specific primers and a HEX-labeled probe binding within the amplicon. Reactions were performed in duplicates by using human leukocyte cDNA (10 ng and 0.2 ng) as template. Measured CT values as well as amplification plots were near identical for all corresponding duplicates, respectively. For each template DNA amount, amplification plots for one of both duplicates is displayed, respectively. Measured PCR efficiency was 98%.
Probe qPCR Master Mix (2x) - Package Contents
- 2x Master Mix containing
- Perpetual Taq DNA Polymerase (Hot Start) ,
- probe optimized reaction buffer,
- dNTPs (dTTP is partially replaced with dUTP).
- Uracil-N-Glycosylase,
- H2O, PCR grade.
- 25 mM MgCl2 solution. If required, please request this component along with your order.
- SG qPCR Master Mix (2x) plus (10x) ROX Solution
- AMV Reverse Transcriptase Native
- Reverse Transcriptases
- Ribonuclease Inhibitor
- RNA Extraction & Purification Kits
qPCR Master Mix Logsheet