Figure 1: OneStep RT-qPCR using the dART 1-Step RT-qPCR Kit. Total RNA from pig liver was used as starting material for quantitative amplification of seven representative housekeeping genes with different transcription abundances. Analyses were performed in quadruplicates. For simplification, only one amplification curve per target gene is pictured. Ct values for each target gene are given as mean value ± standard deviation calculated from all four samples, respectively. To verify the specificity of the reaction, all RT-qPCR products were checked on an agarose gel. The amplification of one single, clean, smear-free and well-formed band with sharp, defined boundaries was confirmed in all cases. One typical amplification curve for each target gene is displayed below. From left to right:

1. Ct 12.6 ± 0.2 - Actin
2. Ct 14.2 ± 0.5 - GAPDH
3. Ct 15.3 ± 0.3 - CD 48
4. Ct 18.0 ± 0.1 - Tubulin
5. Ct 19.8 ± 0.1 - Arginase I
6. Ct 23.1 ± 0.1 - IL3
7. Ct 24.6 ± 0.2 - Cyt 450

SG 1-Step RT-qPCR Master Mix - Package Contents

• Enzyme Master Mix with
  • a reverse transcriptase, dedicated and optimized for RT-qPCR,
  • Perpetual Taq DNA polymerase (Hot Start by monoclonal antibody) ,
  • ribonuclease inhibitor ,
  • and SYBR Green I dye,
• qRT-PCR Master Buffer (2x), a RT-qPCR reaction buffer with dNTPs
  (dTTP is partially replaced with dUTP),
• thermolabile Uracil-N-glycosylase (UNG), provided in a separate tube for optional application,
• H₂O, nuclease free.

Optionally supplied, if needed:

• 25 mM MgCl₂ solution. If required, please request this component along with your order.

Related Products

• SG qPCR Master Mix (2x) plus ROX Solution
• dART cDNA Synthesis Kit with all required and specifically adjusted components for Reverse Transcription
• Reverse Transcriptases
• Ribonuclease Inhibitor
• RNA Extraction & Purification Kits

Additional Resources

 qPCR Master Mix Logsheet

 RT Logsheet