HomeOnline CatalogueqPCR / Real Time PCR KitsNon-Specific Detection: Intercalating Dye qPCR (e.g. Sybr Green) SG 1-Step RT-qPCR Master Mix SG 1-Step RT-qPCR Master Mix

SG 1-Step RT-qPCR Master Mix

Sensitive, high precision one step RT-qPCR master mix for quantitative real time qPCR. Intercalating dye / SybrGreen. Enables direct usage of RNA as template. No ROX.

Detailed Product Description
 English Version
 Deutsche Version

Quantity Package Cat-No. Price in €
25 reactions (25 μl each)E0810-0128.00
100 reactions (25 μl each)E0810-02110.00

Figure 1: OneStep RT-qPCR using the dART 1-Step RT-qPCR Kit. Total RNA from pig liver was used as starting material for quantitative amplification of seven representative housekeeping genes with different transcription abundances. Analyses were performed in quadruplicates. For simplification, only one amplification curve per target gene is pictured. Ct values for each target gene are given as mean value ± standard deviation calculated from all four samples, respectively. To verify the specificity of the reaction, all RT-qPCR products were checked on an agarose gel. The amplification of one single, clean, smear-free and well-formed band with sharp, defined boundaries was confirmed in all cases. One typical amplification curve for each target gene is displayed below. From left to right:

  1. Ct 12.6 ± 0.2 - Actin
  2. Ct 14.2 ± 0.5 - GAPDH
  3. Ct 15.3 ± 0.3 - CD 48
  4. Ct 18.0 ± 0.1 - Tubulin
  5. Ct 19.8 ± 0.1 - Arginase I
  6. Ct 23.1 ± 0.1 - IL3
  7. Ct 24.6 ± 0.2 - Cyt 450

SG 1-Step RT-qPCR Master Mix - Package Contents
Optionally supplied, if needed:
  • 25 mM MgCl2 solution. If required, please request this component along with your order.

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Additional Resources

 qPCR Master Mix Logsheet

 RT Logsheet