1-Step RT-PCR Kit
Convenient one-step cDNA synthesis kit, optimized for sensitive and highly specific RT.
Detailed Product Description
English Version
Deutsche Version
The NG dART OneStep RT-PCR Kit is a convenient system for sensitive one-single-tube RT-PCR reaction setups. It contains an optimized master enzyme mix including the highly processive NG dART reverse transcriptase, high fidelity OptiTaq DNA polymerase (with proofreading activity) and an unique RNase inhibitor, which offers optimal protection of precious RNA not only under ambient conditions but as well at elevated temperatures.
The master mix buffer is an optimized 2x buffer including dNTPs, stabilizers and reaction enhancers.
Optimized towards high efficiency amplification of DNA starting from any RNA, the one-step process proceeds with high specificity and sensitivity. The kit is designed for analytic as well as for cloning purposes.
All required enzymes, optimized buffers, RNase free water and high quality dNTPs are supplied along with the product package.
1-Step RT-PCR Kit - Package Contents
RT Logsheet
PCR Logsheet
The master mix buffer is an optimized 2x buffer including dNTPs, stabilizers and reaction enhancers.
Optimized towards high efficiency amplification of DNA starting from any RNA, the one-step process proceeds with high specificity and sensitivity. The kit is designed for analytic as well as for cloning purposes.
All required enzymes, optimized buffers, RNase free water and high quality dNTPs are supplied along with the product package.
1-Step RT-PCR Kit - Package Contents
- Master Enzyme Mix including
- 2x Master Buffer Mix, including high quality dNTPs,
- and RNase-free water.
- dART RT (Reverse Transcription) Kit, no thermostable DNA polymerase included
- dART 2-Step RT-PCR Kit, complete with OptiTaq DNA Polymerase and PCR Protocol
- RNA Extraction & Purification Kits
- SG qPCR Master Mixes (2x) for qPCR / RealTime PCR
- EURx PCR / DNA CleanUp Kit for purification of PCR products
RT Logsheet PCR Logsheet