Perpetual Taq Master Mix
"Hot Start" Taq DNA polymerase, MAB inhibited.
- Fluorescent PCR methods, genotyping, diagnostic PCR.
- No "proofreading".
- Convenient, stable master mix.
- Contains separate, optional Color Load solution for direct gel loading.
Detailed Product Description
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Application Examples
Figure 1: Comparisonal HotStart PCR amplification of an 1.1 kb amplicon (human ccr5 gene) with Perpetual Taq DNA Polymerase (Cat. No. E2700, non-master-mix format)) and ready-to-use Perpetual Taq PCR Master Mix (Cat. No. 2740). Molecular weight marker MW (Perfect™ 1kb DNA Ladder). Each PCR reaction: 1.25 U Perpetual Taq DNA Polymerase, 0.2 mM dNTPs; 50 µl reaction volume. Lanes 2-3: EURx Perpetual Taq DNA Polymerase (non-master-mix format); 4-5: EURx Perpetual Taq PCR Master Mix (after 25 freeze-thaw cycles, to demonstrate tolerance for repeated freezing and thawing), 6-7: EURx Perpetual Taq PCR Master Mix (after 25 freeze-thaw cycles) plus optional Color Load (for direct post-PCR gel loading).
Figure 2: Sensitive HotStart PCR amplification with Perpetual Taq DNA Polymerase (Cat. No. E2700) in a size range between ~1 and 15 kb. Molecular weight marker M1 (Perfect™ 1kb DNA Ladder). Lanes 1.1 to 15 kb: PCR amplification reactions (each reaction 1.25 U EURx Perpetual Taq DNA Polymerase, Pol Buffer B, 0.2 mM dNTPs; 50 µl reaction volume).
Perpetual Taq PCR Master Mix (2x) - Package Contents
PCR Master Mix Logsheet
Related Articles
Figure 1: Comparisonal HotStart PCR amplification of an 1.1 kb amplicon (human ccr5 gene) with Perpetual Taq DNA Polymerase (Cat. No. E2700, non-master-mix format)) and ready-to-use Perpetual Taq PCR Master Mix (Cat. No. 2740). Molecular weight marker MW (Perfect™ 1kb DNA Ladder). Each PCR reaction: 1.25 U Perpetual Taq DNA Polymerase, 0.2 mM dNTPs; 50 µl reaction volume. Lanes 2-3: EURx Perpetual Taq DNA Polymerase (non-master-mix format); 4-5: EURx Perpetual Taq PCR Master Mix (after 25 freeze-thaw cycles, to demonstrate tolerance for repeated freezing and thawing), 6-7: EURx Perpetual Taq PCR Master Mix (after 25 freeze-thaw cycles) plus optional Color Load (for direct post-PCR gel loading).
Figure 2: Sensitive HotStart PCR amplification with Perpetual Taq DNA Polymerase (Cat. No. E2700) in a size range between ~1 and 15 kb. Molecular weight marker M1 (Perfect™ 1kb DNA Ladder). Lanes 1.1 to 15 kb: PCR amplification reactions (each reaction 1.25 U EURx Perpetual Taq DNA Polymerase, Pol Buffer B, 0.2 mM dNTPs; 50 µl reaction volume).
Perpetual Taq PCR Master Mix (2x) - Package Contents
- Perpetual Taq PCR Master Mix (2x)
- PCR Water (Nuclease Free)
- 10x Color Load (Optinal reaction component: Coloured reaction buffer, for direct gel loading)
- Optionally supplied, if needed:
- 25 mM MgCl2 solution. If required, please request this component along with your order.
PCR Master Mix LogsheetRelated Articles