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Polymerase X — Hybrid DNA Polymerase

Extremely accurate, fast and efficient synthetic DNA Polymerase. For PCR reactions demanding maximum precision and excellent yield.

Detailed Product Description
 English Version
 Deutsche Version

Quantity Package Cat-No. Price in €
100 uE2950-0130.00
500 uE2950-02123.00
100 u / Kit (+ dNTPs)EK2950-0132.00
500 u / Kit (+ dNTPs)EK2950-02135.00

Melting Temperature (Tm) Calculation

Calculate Tm's using the base-stacking method (nearest-neighbor method).
Default parameters are: 500 nM primer concentration, 50 mM salt concentration, 1,5 mM Mg2+ concentration.

Primer (6-50 bases):
Basic Tm
Degenerated nucleotides are allowed
Base-Stacking Tm
Degenerated nucleotides are NOT allowed
Primer concentration: nM
Salt concentration: mM
Mg2+ concentration: mM

Source code is freely downloable at biophp.org
Application Example

Figure: Proofreading PCR amplification with Polymerase X - Hybrid DNA Polymerase (Cat. No. E2950) in a size range between ~1 and 12 kb. Molecular weight marker MW (PerfectPlus™ 1kb DNA Ladder). Lanes 1.1 to 12 kb: PCR amplification reactions (each reaction 1.0 U EURx Polymerase X - Hybrid DNA Polymerase, Pfu Buffer, 0.25 - 0.3 mM dNTPs; 50 µl reaction volume). For further reaction parameters see manual.

Polymerase X – Hybrid DNA Polymerase - Package Contents
  • Polymerase X - Hybrid DNA Polymerase
  • 10x Hybrid Buffer
  • 25 mM MgCl2 solution
Additional Resources

 PCR Logsheet