Pfu DNA Polymerase
Top quality special application Pfu DNA polymerase, 5.0 u/µl
Detailed Product Description
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Application Example
Figure: Proofreading PCR amplification with Pfu DNA Polymerase (Cat. No. E1114) in a size range between ~1 and 8.0 kb. Molecular weight marker M1 (Perfect™ 1kb DNA Ladder). Lanes 1.1 to 20 kb: PCR amplification reactions (each reaction 2.5 U EURx Pfu DNA Polymerase, Pfu Buffer, 0.25 - 0.3 mM dNTPs; 50 µl reaction volume). For further reaction parameters see manual.
Pfu DNA Polymerase - Package Contents
PCR Logsheet
Figure: Proofreading PCR amplification with Pfu DNA Polymerase (Cat. No. E1114) in a size range between ~1 and 8.0 kb. Molecular weight marker M1 (Perfect™ 1kb DNA Ladder). Lanes 1.1 to 20 kb: PCR amplification reactions (each reaction 2.5 U EURx Pfu DNA Polymerase, Pfu Buffer, 0.25 - 0.3 mM dNTPs; 50 µl reaction volume). For further reaction parameters see manual.
Pfu DNA Polymerase - Package Contents
- Pfu DNA Polymerase
- 10x Pfu Buffer
- 25 mM MgSO4 solution
- Same as Pfu DNA Polymerase, plus ultrapure dNTP solution
- PfuPlus! DNA Polymerase is a Pfu formulation targeted towards long PCR amplicons (up to 20 kb) from episomal DNA. PfuPlus! is the enzyme of choice for Site Directed Mutagenesis.
- Polymerase X - Hybrid DNA Polymerase is a fast and extremely precise enzyme for amplification of both chromosomal and episomal DNA.
PCR Logsheet