Taq DNA Polymerase

Top quality general application Taq DNA polymerase.

• General & diagnostic PCR.
• High purity.
• No "proofreading".
• Separate enzyme and 10x buffers.
• Contains both, non-colored and colored 10x PCR buffers for direct gel loading

  Detailed Product Description
   English Version
   Deutsche Version

  Quantity	Package	Cat-No.	Price in €
  	200 u	E2500-01	29.00
  	500 u	E2500-04	64.00
  	1000 u	E2500-02	111.00
  	5000 u	E2500-03	550.00
  	200 u / Kit (+ dNTPs)	EK2500-01	37.00
  	500 u / Kit (+ dNTPs)	EK2500-04	83.00
  	1000 u / Kit (+ dNTPs)	EK2500-02	150.00
  	5000 u / Kit (+ dNTPs)	EK2500-03	743.00
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  Pure. Performant. No contaminating DNA.
  
  

  

  
  Figure 1: Taq DNA Polymerase - Package Contents

  • Taq DNA Polymerase
  • 10x Buffer Pol A (Optimization reaction buffer without MgCl₂)
  • 10x Buffer Pol B (Standard reaction buffer containing MgCl₂)
  • 10x Buffer Pol C (Purple coloured reaction buffer, for direct gel loading)
  • 25 mM MgCl₂ solution

  Taq DNA Polymerase - Kit Package Contents

  • Same as Taq DNA Polymerase, plus ultrapure dNTP solution

  Application Example
  
  

  

  
  Figure 2: PCR amplification with Taq DNA Polymerase (Cat. No. E2500) in a size range between ~1 and 15 kb. Molecular weight markers MW1 (→ Perfect™ 1kb DNA Ladder), MW2 (Lambda-DNA [Phage λ, GenBank J02459] / → HindIII). Lanes 1.1 to 15 kb: PCR amplification reactions (each reaction 1.25 U EURx Taq DNA Polymerase, Pol Buffer B, 0.2 mM dNTPs; 50 µl reaction volume).
  
  Features

  • All-Purpose Taq: For all PCR applications not requiring "HotStart". Great all-purpose DNA polymerase for general PCR applications.
  • High Quality: High purity and efficient enzymatic activity as a result of thorough but gentle enzyme purification.
  • Strict Quality Control: Optimized towards high enzyme quality: Taq DNA polymerase is continuously and extensively monitored.
    • No false positives: No detection of PCR-amplificable DNA, as tested in 40+ cycle PCR reactions with various amplification targets of human, eukaryotic, bacterial and viral origin (including bacterial 16S rRNA genes).
    • Extreme Purity: PAGE gel analysis of purified enzyme shows presence of just one single band, as one would expect for a >95% pure preparation.
    • Devoid of Nucleases and Proteases: Sensitive enzymatic tests reveal absence of any contaminating nucleases or proteases.
    • Strict quality controls ensure continuous reproducible high enzyme purity and performance, as opposed to certain infamous "crude extracts with intrinsic DNA polymerase activity" offered by some third parties.
    • Precise Conformity with Unit Specification: Strict and precise adjustment of enzymatic activity towards the regular, commonly accepted unit definition. One unit of enzyme is exactly one unit - and not less. At 74°C, one unit catalyzes the incorporation of precisely 10 nmoles of dNTP into acid-insoluble material within 30 min, no more, no less.
  • Excellent Price-Performance ratio: Good value for money.
  • Recombinant: Optimized, improved performance as compared to → Native Taq DNA Polymerase [Cat. No. E2504]).
  • All Buffers Inclusive: Ships complete with non-colored plus with colored buffers for direct gel loading.
  • Enzyme Properties: Intrinsic exonuclease activities: 5'-3' present, 3'-5' none. No proofreading activity.
  • Cloning: Enables both TA- and blunt end cloning.
  • Also available as: → Taq PCR Master Mix [Cat. No. E2520]).

  Additional Resources
  
   PCR Logsheet
  
   How to trigger Reverse Transcriptase Activity of Taq DNA Polymerase (Contributed Protocol)